We have reported that a combination o…
Objective:this study was

to discuss the effect of warmedinfusion on extensive bums complicated with low bodytemperature during its shock resuscitation stage.methods:we used rabbit 35%TBSAⅢburn model to undergoresuscitation.Fluids warmed to 38-39℃were applied inexperiment group.Fluids at room tem…
背景和目的:丹酚酸B(SA-B)是丹参的水溶性成份之一(C36H30O16,Mr 718)。临床观察发现,SA-B有抗慢性乙型肝炎肝纤维化作用;实验研究证实,SA-B能抑制TGF-β1刺激的肝星状细胞活化与Smad2磷酸化。肝星状细胞活化是肝纤维化细胞学基础,TGF-β1是重要的促肝纤维化因子,可通过TGF-β受体及其Smads蛋白通路促进肝星状细胞活化。本研究主要目的在于探讨SA-B干预肝星状细胞中TGF-β1信号转导的抗肝纤维化作用。方法:分离培养原代肝星状细胞(HSC),于培养4d(细胞处于中间活化状态)时,随机分为正常培养、TGF-β1刺激组、抑制剂对照组(10μM Z VAD FMK SB-431542…
We aimed to study whether the PPAR-g agonist pioglitazone (PIO) induces apop-tosis in vascular smooth muscle cells (VSMC) from diabetic patients and its relationship with TGF-b. Methods: VSMC were isolated by explants from internal mammary arteries. Apoptosis induced by PIO 100mcM was analyzed b…
Hyaluronic acid (HA) is a key glycosaminoglycan (GAG) mediating vascular smooth muscle (VSMC) proliferation and migration. We investigated

the effect of TGF – betal. on HA turnover in primary human VSMC obtained from the pulmonary artery. Cells were incubated with TGF- betal for 6, 12 and 24 h. …
Aim:To investigate the mechanism selleck怎么样

selleck好不好

selleck怎么样 of UVB inducing apoptosis in HaCaT cells via iNOS/NO/TGF-β/Smads pathway

and Polypeptide 或者 from Chiamys farreri(PCF) protecting HaCaT cells from UVB by 20 mJ/cm~2 UVB-induced apoptotic model of HaCaT cells and iNOS transient transfected HaCaT cells.Methods:UVB-induc…
目的Rho激酶(ROCK)活化在压力超负荷介导的左心室重构中发挥重要作用,其潜在的病理分子机制尚不明确。在本研究中,我们发现TGF-β1可诱导ROCK活化,并通过抑制内源性BMP-2加重心肌纤维化。因此,利用外源性补充BMP-2分子观察是否有效改善压力超负荷下TGF-β1引起的心肌纤维化,并阐明内在的病理机制。方法通过机械牵张心肌细胞,在不同时间段(0、12、24、48 h)观察细胞BMP-2、TGF-β1以及ROCK蛋白的表达;
Selenium has been reported to possess potent anti-oxidant, anti-hyperglycemic and anticarcinogenic propertiesHowever, the precise biological role of selenium in formation of skin fibril remains unknownSelenium exists in various forms such as selenate, selenite, and methylseleninic acidAmong them, we…
目的SB431542是TGF8的Ⅰ型±受体的特异性抑制剂,利用它阻断TGF-β1/SMADs信号通路对严重烧伤后促炎性细胞因子IL-1β、IL-6、表达的影响。方法雄性SD造成后躯干30%TBSAⅢ。烧伤,伤后按ParkLand公式补液抗休克。分为对照组(单纯烧伤)和抑制剂组(烧伤前1h腹腔内注射SB431542,剂量2.53ug/g,烧伤同对照)。于烧伤后2h、8h采集血清,ELISA法测定血清IL-1β、IL-6、TNF-α水平。结果伤后2h,8h血清IL-1β、IL-6、TNFα水平抑制组均显著低于对照组(P
目的探讨TGF-β/Smads信号转导通路在C-57小鼠皮肤组织损伤愈合过程中的作用。着重研究其对重上皮化的影响。方法动物分组:1、正常组(N)。2、正常鼠应用受体抑制剂SB-431542组(NS)。3、Smad4基因敲除组(S)。4、Smad4基因敲除应用TGF-β受体抑制剂SB-431542(S S)。每组小鼠3只。1、3组小鼠造成Ⅱ度烫伤,模型。2、4组小鼠于烫伤前30分钟给予腹腔应用SB-431542(5X10-8/g),并每日给药一次,持续5天,观察小鼠烫伤后愈合速度;1组与3组小鼠于伤后5天从创伤边缘取材,剪程1mmX2mm组织块,于无血清培养基中培养,1组分NT和NST、亚组,3…